Detection of Acetaminophen and Its Glucuronide in Fingerprint by SALDI Mass Spectrometry Using Zeolite and Study of Time-Dependent Changes in Detected Ion Amount

The ingredients of an antipyretic (acetaminophen, AAP) and their metabolites excreted into fingerprint were detected by surface-assisted laser desorption ionization (SALDI) mass spectrometry using zeolite. In the taken 4 h after AAP ingestion, not only but also glucuronic acid conjugate (GAAP), caffeine (Caf), ethenzamide (Eth), salicylamide (Sala; a metabolite Eth), urea detected. Fingerprints collected over time to determine how amounts its changed with time, dependence peak intensities protonated GAAP was measured. It found that increase [GAAP+H]+ started later than [AAP+H]+ peak, reflecting metabolism AAP. Both reached maximum concentrations approximately 3 from body half-life 3.3 h. addition, preservation confirmed optical microscopy, shape retained even irradiation fingerprint. Our method may be used in analysis.